بهداشت و بیماری‌های عفونی دام

بهداشت و بیماری‌های عفونی دام

اثرات ضدباکتریایی عصاره اتانولی و کلروفرمی ترخون بر عوامل بیماریزای باکتریهای اشرشیا کلی و سودوموناس آئروژینوزا

نوع مقاله : مقاله پژوهشی

نویسندگان
صبا هوشیاری 1
عزیزالله ابراهیمی کهریزسنگی 2
محمدرضا محزونیه 1
سمیه شاهرخ شهرکی 3
1 بخش پاتوبیولوژی دانشکده دامپزشکی دانشگاه شهرکرد، شهرکرد، ایران
2 بخش پاتوبیولوژی دانشکده دامپزشکی دانشگاه شهرکرد. شهرکرد. ایران
3 استادیار بخش پاتوبیولوژی دانشکده دامپزشکی دانشگاه شهرکرد، شهرکرد
10.22034/jahid.2026.2081753.1066
چکیده
زمینه و اهداف: برخی از گیاهان دارویی دارای ترکیباتی هستند که فعالیت ضدمیکروبی علیه باکتری‌های بیماری‌زا دارند. برخی از باکتریهای بیماریزا مانند سودوموناس آئروژینوزا هرساله به آنتی بیوتیکهای موجود مقاوم میشوند و درمان عفونتهای چنین باکتریهایی با چالش مواجه شده است و نیز باکتری اشرشیا کلی عفونتهای متعددی در بدن ایجاد میکنند و هرساله باعث مرگ و میر افراد زیادی میشوند. فلذا با توجه به اهمیت این موضوع، این تحقیق به منظور بررسی خواص ضدباکتریایی عصاره‌های اتانولی و کلروفرمی گیاه ترخون (Artemisia dracunculus ) که در طب سنتی استفاده های زیادی دارد ، علیه برخی از فاکتورهای بیماری‌زای (بیوفیلم، سوارمینگ، تشکیل پیوسیانین و همولیزین) باکتریهای اشریشیا کلی و سودوموناس آئروژینوزا که 2 باکتری مهم و خطرناک است ، انجام شد.
مواد و روش‌ کار: عصاره‌های اتانولی و کلروفرمی گیاه با استفاده از کلروفرم و اتانول ۸۵٪ تهیه شد. از روش ماکرودایلوشن براث برای تعیین حداقل غلظت مهارکنندگی (MIC) عصاره‌ها استفاده شد، سپس تأثیر عصاره‌ها بر برخی از فاکتورهای بیماری‌زایی مانند تشکیل بیوفیلم ، همولایزین، پیوسیانین و سوآرمینگ در باکتریهای موردنظر، ارزیابی شد.
یافته ها : MICهای به‌دست‌آمده نشان داد که عصاره‌های اتانولی و کلروفرمی این گیاهان می‌توانند رشد باکتری‌های مورد بررسی را مهار کنند. همچنین تولید برخی از فاکتورهای بیماری‌زایی مانند همولیزین، پیوسیانین، تشکیل بیوفیلم و سوارمینگ را تحت تأثیر قرار میدهند. در باکتری سودوموناس آئروژینوزا تولید پیوسیانین و همولایزین توسط عصاره اتانولی ترخون کاهش یافت و تولید بیوفیلم و سوارمینگ توسط هردوعصاره کاهش یافت. و در باکتری اشرشیا کلی، تولید همولایزین و بیوفیلم توسط هر دو عصاره اتانولی و کلروفرمی کاهش یافت و در تست سوارمینگ ، عصاره کلروفرمی توانست حرکت جمعیت باکتری اشرشیا کلی را کاهش دهد.
نتیجه گیری : عصاره‌های گیاهی می‌توانند تولید برخی از فاکتورهای بیماری‌زایی باکتریایی در دو باکتری را مهار کنند و احتمالا" در ترکیب با آنتی‌بیوتیک‌ها برای مقابله با مقاومت و عوارض جانبی استفاده شوند.
کلیدواژه‌ها
بیوفیلم
سوارمینگ
همولایزین
پیوسیانین
سودوموناس آئروژینوزا
اشریشیا کلی
موضوعات

عنوان مقاله English

Antibacterial effects of Artemisia dracunculus extracts against Pseudomonas aeruginosa and Escherichia coli virulent factors

نویسندگان English

saba Hooshyari 1
Azizollah Ebrahimi Kahrizsangi 2
mohammadreza mahzoonieh 1
somayeh shahrokh shahraki 3
1 Department of Pathobiology, faculty of veterinary .Shahrekord University, Shahrekord, .Iran
2 Department of Pathobiology Shahrekord University .Shahrekord. iran
3 Department of Pathobiology, Shahrekord University, Shahrekord, Iran
چکیده English

Background: Some medicinal plants contain compounds that have antimicrobial activity against pathogenic bacteria. Some pathogenic bacteria, such as Pseudomonas aeruginosa, become resistant to existing antibiotics every year, making treatment of infections caused by such bacteria a challenge. Escherichia coli bacteria also cause numerous infections in the body and cause many deaths every year. Therefore, considering the importance of this issue, this study was conducted to investigate the antibacterial properties of ethanolic and chloroform extracts of the tarragon plant (Artemisia dracunculus), which has many uses in traditional medicine, against some pathogenic factors (biofilm, swarming, pyocyanin and hemolysin formation) of Escherichia coli and Pseudomonas aeruginosa, which are two important and dangerous bacteria.
Material and Methods: Ethanolic and chloroform extracts of the plant were prepared using chloroform and 85% ethanol. Broth macrodilution method was used to determine the minimum inhibitory concentration (MIC) of the extracts, then the effect of the extracts on some pathogenic factors such as biofilm formation, hemolysin, pyocyanin and swarming in the target bacteria was evaluated.
Results: The obtained MICs showed that ethanolic and chloroformic extracts of these plants can inhibit the growth of the studied bacteria. They also affect the production of some virulent factors such as hemolysin, pyocyanin, biofilm formation and swarming. In Pseudomonas aeruginosa, pyocyanin and hemolysin productions were reduced by ethanolic extract of tarragon, and biofilm production and swarming were reduced by both extracts. In Escherichia coli, hemolysin and biofilm productions were reduced by both ethanolic and chloroformic extracts, and in the swarming test, chloroformic extract was able to reduce the movement of Escherichia coli population.
Conclusion: Plant extracts can inhibit the production of some bacterial pathogenic factors and may be used in combination with antibiotics to combat resistance and side effects.

کلیدواژه‌ها English

biofilm
swarming,
hemolysin,
pyocyanin,
Pseudomonas aeruginosa
Escherichia coli
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بهداشت و بیماری‌های عفونی دام
دوره 3، شماره 1
تیر 1405
صفحه 82-87