Abstract:
Background and Objective: For many years, doctors and medicine have been thinking about shortening the wound healing time, finding a way to heal the wound as quickly as possible. This study was conducted in order to investigate the healing properties of wild parsnip leaf extract ointment in full-thickness wounds of rats and compare it with phenytoin ointment.
Methods: In this study, after induction of anesthesia by Ketamine and Xylazine, a wound was created on the back of each of the rats. Classification of wound healing in 4 groups including: first group: no treatment (GA), second group: treatment with Oserin (ointment base) (GB), third group: treatment with Mespilus germanica leaf extract ointment (GC) and fourth group: They were treated with phenytoin ointment (GD). Wounds were treated and measured by analyzing images using Scion-Image software. For microscopic studies, samples were taken from the healing site on days 3, 7, 9, 12 and 15. Wound healing was noted for all treatment groups. The data was analyzed with SPSS22 software. ANOVA results showed that there is a significant difference between the treated groups (GA, GB, GC and GD) (p<0.05).
Results: The results showed that there is a significant difference between day 7 and other days (3, 9, 12 and 15) (p<0.05). Also, the effectiveness of the drugs on the 7th day showed that the ointment of extracted M. germanica leaf had a more effective and stronger effect on wound healing than the drugs Oserin and Phenytoin (the Sig obtained for the 7th day was less than 0.05). It should be noted that there was no significant difference between Oserin and Phenytoin (p>0.05). Histopathological results showed that the effectiveness of M. germanica leaf extract on day 7 was more than other drugs (phenytoin and oserin).
Conclusion: Today, in the field of veterinary medicine, significant progress has been made in the field of wound healing and surgical procedures with minimal wound and damage. However, the issue of healing the wound as quickly as possible with less infection and a better healing process is very important and vital. According to the research conducted in this study, it was concluded that there is no significant difference between any of the treatments.
Key words: Oserin wild parsnip, phenytoin ointment, Wound healing