Animal Health and Infectious Diseases

Animal Health and Infectious Diseases

Molecular detection of canine pathogenic leptospira using polymerase chain reaction method

Document Type : Original Article

Authors
Shabnam anari 1
Shahriyar Mehrabi 2
1 Lorestan University.Faculty of veterinary Medicine
2 DVSc Graduate of Larg Animal Internal Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahavz, Iran
10.22034/jahid.2024.714485
Abstract
This is an overview of the prevalence of Leptospira in Khorramabad using PCR technique. Leptospirosis is a common bacterium that is common among humans and has a wide range of domestic and wild species (cows, sheep, goats, horses, pigs, goats, etc.). And humans are involved. The main reservoirs of the disease are rodents and semi-wild animals in the suburbs and villages, including dogs. In order to carry out this study, which was carried out in the period of 2008-2009, 60 blood samples were taken from referred dogs. The samples were kept in the freezer until molecular tests were performed. To perform DNA extraction from the samples, the DNA extraction kit of Fargen company, Taiwan was used. srRNA 16 and LipL32 gene primers were used for molecular tracking of Leptospira. Out of a total of 60 blood samples studied, 6 samples (10%) were infected with Leptospira bacteria. conclusion; Considering that no study has been conducted on leptospirosis in dogs in Khorram Abad region, so this study can be an important step towards determining the prevalence of leptospirosis in this region. Health recommendations to vaccinate dogs to prevent disease transmission to humans should be submitted to the regional veterinary department. The special situation of Lorestan province and the irregular entry and exit of livestock and their smuggling to neighboring countries and the lack of quarantine of livestock seem to be necessary to conduct a comprehensive study on the prevalence of Leptospira in Lorestan province and especially Khoramabad city. Consider all dimensions.
Key words: leptospirosis, Khorramabad region, dog, polymerase chain reaction
Keywords
Leptospirosis
Khorramabad region
Dog
polymerase chain reaction
Subjects
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  • Receive Date 04 April 2024
  • Revise Date 25 May 2024
  • Accept Date 09 June 2024
  • First Publish Date 22 July 2024
  • Publish Date 22 June 2025