Mesenchymal Stem Cells (MSCs) have gained attention in recent years as an innovative approach for treating diseases and repairing tissues in the field of veterinary medicine. These cells, characterized by their self-renewal capacity and ability to differentiate into various types of connective tissue cells, have become a promising and effective tool in clinical therapies, particularly due to their immunomodulatory properties. Mesenchymal stem cells are categorized into different groups based on their differentiation potential and their source. Among the various sources of stem cells, adult mesenchymal stem cells currently appear to be the most suitable for therapeutic purposes, as they have simple isolation and culture techniques, and these cells have a very limited teratogenic risk compared to pluripotent stem cells. Significant advances have been made in the development of safe and effective stem cell therapies in recent years. Mesenchymal stem cells can aid in wound healing and scar reduction, showing considerable potential in the repair and regeneration of various tissues in animals. The mechanism of action of these cells includes the release of growth factors, modulation of immune response, and promotion of angiogenesis. Thus, this review study aims to explore the capabilities of mesenchymal stem cells as a powerful therapeutic method in treating disorders and diseases of various tissues, including the skin, musculoskeletal system, digestive tract, and eyes in different animals. The purpose of the present study as a comprehensive review of this novel treatment and can act as a guide for researchers and veterinarians to optimally utilize the capabilities of mesenchymal stem cells in treating diseases and tissue injuries in various livestock.