Animal Health and Infectious Diseases

Animal Health and Infectious Diseases

Application of magnetic chitosan nanostructures in immobilization of bacterial alpha-amylase enzyme

Document Type : Original Article

Authors
Saideh Afrisham 1
Arastoo Badoei-Dalfard 2
Seyed ahmad Ataei 3
Shirin Mohammadipour 1
1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
2 Department of Biology, Faculty of sciences, Shahid Bahonar University of Kerman, Kerman, Iran
3 Department of Chemical Engineering, Faculty of Technology and Engineering, Shahid Bahonar University of Kerman
10.22034/jahid.2025.2055099.1056
Abstract
Alpha-amylases belong to the endoamylase family, which perform the primary hydrolysis of starch to smaller oligosaccharides by breaking the (4→1)α bond. Among the different types of enzymes, alpha-amylases constitute an important group with wide applications in various industries. The use of free enzymes in various industries has limitations, including: high cost, lower stability, poor reusability, and difficulty in using them in continuous reactors. These limitations can be minimized by immobilizing the enzyme. Enzyme immobilization can be performed on various substrates. In the meantime, enzyme immobilization on nano-surfaces, especially magnetic nanoparticles, has received much attention due to their low toxicity, reusability of the enzyme along with increased stability and robust performance under various conditions. In this study, alpha-amylase enzyme immobilization was performed using Fe2O3 nanoparticles on a chitosan polymer substrate. For the initial production of the enzyme, an overnight culture of Bacillus mujavensis was inoculated into a specific liquid medium and incubated for 48 hours. The obtained enzyme was concentrated using ammonium sulfate and then dialyzed. The dialyzed enzyme was bound to Fe2O3 nanoparticles in a chitosan substrate in the presence of 50% glutaraldehyde (binding agent). The activity and stability of the enzyme immobilized with nanoparticles were measured at 540 nm and were found to be 53 and 48% higher than the free enzyme, respectively. The concentration of the immobilized enzyme was also estimated using a standard curve of 25 μg bovine serum albumin.
Keywords
Alpha-amylase
immobilization
magnetic nanoparticles
chitosan
activity
Subjects
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Animal Health and Infectious Diseases
Volume 2, Issue 2 - Serial Number 3
December 2025
Pages 46-50

  • Receive Date 03 October 2025
  • Revise Date 20 November 2025
  • Accept Date 07 December 2025
  • First Publish Date 07 December 2025
  • Publish Date 22 December 2025